期刊论文

中文

应用化工

1671-3206

2012

41

11

1999-2001,2003

10.16581/j.cnki.issn1671-3206.2012.11.010

基金项目：农业部农残试验基金项目（2010H216）

本校为第一机构

建立了测定土壤和烟叶中精喹禾灵残留量的高效液相色谱分析方法。土壤和烟叶样品中精喹禾灵用丙酮提取,经弗罗里硅土净化后,采用XB-C_(18)柱(150 mm×4.6 mm,5.0 μm),以甲醇-水(80∶20,v∶v)为流动相,流速为0.6 mL/min,柱温为25 ℃,检测波长为236 nm,采用外标法定量。精喹禾灵的浓度在0.05~5.00 mg/L范围内时,其线性关系良好(R~2=0.997 4),回收率在85.47%~106.63%,相对标准偏差在0.71%~4.26%。该方法操作简便,灵敏度、准确度和精密度高,能满足农药残留量分析与检测技术的要求。

An analytical method was established to determine the residue of quizafop-p-ethyl in soil and tobacco by high performance liquid chromatography. The residues in soil and tobacco was extracted with acetone,and the extract was cleaned up by a Florisil chromatography column. The separation was per- formed on a C18 column（ 150 mm ×4.6 mm,5.0 μm）at 25 ℃ by HPLC with an ultraviolet dector（UVD） at 236 nm,and methanol-water（80：20,v：v） as the mobile phase at a flow rate of 0. 6 mL/min. The quan- tification was performed by external standard. ...