The reaction kinetics for synthesis of capsinoids by lipase-catalyzed transesterification of vanillyl alcohol and methyl nonanoate in acetone was investigated in this work.The concentration of capsinoids obtained in the reaction was detected by HPLC and then the initial velocity of the synthesis reaction was calculated.The inhibiting test of product was carried out with methanol added.The results revealed that the enzymatic-synthesis reaction follows the Ping-Pang reaction mechanism.The kinetic equation with corresponding Vmax,KmA and KmB was inferred by the double reciprocal plot.The fitting ...
