K326 was used as the material to study the effect of one-time and several-times harvest on the growth and nicotine content of the upper leaves in Changsha,Hunan in 2005. The results showed that harvest way could affect the biological characters of the upper leaves and regulate the accumulation of nicotine in different positions of leaves in a way. Increase of harvest times of upper leaves and decrease of number of reserved leaves were disadvantageous to control nicotine content in the upper leaves. Harvesting the upper 6 leaves in one time could effectively control the problem of exorbitant ni...