期刊论文

中文

作物研究

1001-5280

2010

24

1

30-35

10.16848/j.cnki.issn.1001-5280.2010.01.010

基金项目：科技部国家科技基础条件平台建设项目（2004DKA30430）

本校为第一机构

2009年以16个鱼腥草为材料,通过正交设计研究了鱼腥草的SRAP-PCR反应体系和扩增体系,并运用SRAP标记技术构建了16个鱼腥草材料的SRAP-PCR图谱,同时应用DPS分析软件构建了UPGMA聚类图.结果表明:(1)最佳反应体系为总体积25 μL中含40 ng DNA,0.1 mmol/L dNTPs,2.0 mmol/L Mg2+,37.5 ng/μL引物和2 U Taq酶.(2)从360对引物中筛选出条带清晰多态性好的118对,共扩增出7 582个条带,其中多态性条带6 590个,多态率为86.92%.(3)ZY06-028在Me9-GA18扩增产物的250 bp处有特异性缺失带,而ZY06-016 和ZY06-028在550 bp处有特异性缺失带;在Me8-Em10的扩增产物中ZY06-42和ZY06-01分别在300 bp和450 bp处出现特异...

The SRAP amplified and reaction systems were researched and SRAP--PCR map of 16 genotypes of Houttuynia cordata Thunb were constructed and a UPGMA dendrogram was obtained by applying DPS software. The results were as follows： （1） The best reaction system was 40 ng DNA, 0. 1 mmol/L dNTPs, 2. 0 mmol/L Mg2＋, 37. 5 ng/μL primer and 2 U Taq polymerase in 25 μL system. （2） 118 pairs of primers were selected from 360 pairs and 7 582 bands were amplified, of which 6 590 were polymorphic, and the polymorphic percentage of bands was 86. 92%. （3）...