In order to explore the plastid pigment difference of different periods and different leaf position in the late growth stage of tobacco with great aroma in southern Hunan, the pigments content changes and correlation of chlorophyll content and SPAD value on the upper, middle and lower leaves were studied at topping stage, after topping 10 days and 20 days of YY87. The results showed that the chlorophyll a, chlorophyll b and total chlorophyll contents in different leaf positions at the same period were:upper leaf > middle leaf > lower leaves, except that the difference of upper leaves and middl...