期刊论文

中文

作物研究

1001-5280

2014

1

57-62

10.3969/j.issn.1001-5280.2014.01.14

湘潭市科技计划重点项目（ZD20121015）.

本校为第一机构

湘莲腐败病是一种对湘莲危害极其严重的病,发病严重时,可导致莲藕严重减产甚至绝收。本试验从患病的莲藕上完成了对病原菌的分离,并对该致病菌进行了纯培养,从病菌的生物学特质、ITS序列分析及致病性接种鉴定等方面,确定该病菌的病原菌类型为尖镰孢菌。同时应用菌丝体生长速率法,对生产中防治湘莲腐败病的常用化学农药如多菌灵、百菌清等进行室内毒力测定,选择出最适的防治药剂。测试结果显示,不同药剂对病原菌菌丝生长的抑制作用存在明显的差别,抑制效果最明显的为硫磺·多菌灵,抑制中浓度( EC50)为19．87μg/mL；其次为多菌灵,EC50为104．11μg/mL。

Rhizome rot is a serious disease harm to the lotus, and its serious incidence can cause serious decrease of yield of lotus roots. In this test, the separation of pathogenic bacteria from diseased lotus root was finished, and the pathogens were cultured, and the type of the pathogen was Fusarium oxysporum which be determined from the aspects of biological characteristics, ITS sequence analysis and inoculated identification on pathogenicity. At the same time, toxicity determina-tion of commonly used chemical pesticides to control lotus rot diseas...