期刊论文

Breeding New Rice Line with Blast Resistance by Molecular Marker- assisted Selection of the Pi9 Gene

中文

作物研究

1001-5280

2015

29

4

348-351,356

10.3969/j.issn.1001-5280.2015.04.04

3l171526:国家自然科学基金 2014ZX08001-002:转基因生物新品种培育科技重大专项 IRT1239:教育部***和创新团队发展计划项目

本校为第一机构

利用广谱持久抗瘟基因Pi9的内含子序列设计特异功能标记Ins2-1,通过分子标记辅助选择和连续回交育种,改良早籼稻1701的稻瘟病抗性.结果表明:Ins2-1是一个显性分子标记,在Pi9基因供体亲本75-1-127和受体亲本1701之间多态性明显且稳定;Ins2-1标记基因型对稻瘟病抗性表型的选择效率为100％;通过连续回交和自交,获得了含Pi9基因且遗传背景接近受体亲本的BC6 F2群体;通过对BC6F3株系标记基因型和室内接种表型鉴定,筛选出了一个抗病纯系“抗瘟1701”.

To improve blast resistance of the early season indica rice line 1701, a dominant functional DNA marker, Ins2-1, was developed based on the intron sequence of the broad-spectrum blast resistance gene Pi9 and marker-assisted selection breeding practice was implemented in this study. The main results were as follows： Ins2-1 showed stable polymotphism be- tween 1701 and 75-1-127,the Pi9 donor, and the selection efficiency of the marker genotype for blast resistance phenotype was 100%. A BC6F2 population,harboring the Pi9 gene and consecutive back...