期刊论文

中文

作物研究

1001-5280

2018

32

3

202-207

10.16848/j.cnki.issn.1001-5280.2018.03.07

湖南省教育厅科学研究项目（15K060）；

本校为第一机构

根据Gen Bank收录的拟南芥At SPT基因c DNA序列设计引物,采用同源克隆的方法从野生型荠菜（Capsella bursa-pastoris）中RT-PCR扩增出荠菜Cb SPT基因全长c DNA,通过生物信息学分析表明:荠菜Cb SPT基因c DNA序列1191 bp,其中开放阅读框1047 bp,编码348个氨基酸,与拟南芥At SPT基因的核酸序列和氨基酸序列的同源性分别达到98%和93%左右,可以判断为荠菜Cb SPT基因c DNA序列（Cb SPT）。以Ti质粒p WM101为基础,构建了由Ca MV35S启动子调控的Cb SPT基因植物表达载体p WM101-35S::Cb SPT。采用根癌农杆菌介导的浸花序法转化荠菜的同科植物拟南芥,获得了转Cb SPT基因的拟南芥植株。转基因拟南芥心皮果荚...

The primers were designed according to the Arabidopsis At SPT c DNA sequence contained in Gen Bank,with the method of Homology-based cloning,the full-length c DNA of the Cb SPT gene was amplified by RT-PCR in Capsella bursa-pastoris. Bioinformatics analysis showed that： the capsella bursa-pastoris Cb SPT gene c DNA sequence 1191 bp,with 1047 bp open reading frames,coding 348 amino acids,the homology of nucleic acid sequence and amino acid sequence of the At SPT gene of Arabidopsis was 98% and 93% respectively,which could be judged as the Cb SP...