期刊论文

中文

中国兽医学报

1005-4545

2011

31

9

1290-1294

10.16303/j.cnki.1005-4545.2011.09.011

湖南省科技计划资助项目

本校为第一机构

从湖南各地送检至实验室的临床样本中分离到42株革兰氏染色阳性及过氧化氢酶阴性的球菌,其中78.6%来自于肺脏,菌落形态及染色镜检均与粪肠球菌参考株ATCC 29212相似,兰氏分群鉴定97.6%（41/42）为D群,生化特性符合粪肠球菌特征。16SrDNA测序鉴定显示：42个分离株与同步测序的ATCC 29212同源性在99.2%到100%之间,与所选择粪肠球菌参考序列的同源性在98.7%到100%之间,NCBI在线BLAST分析发现42株均与GeneBank收录的粪肠球菌序列同源性最高。湖南分离株16SrDNA序列与E.faecalis参考序列进化关系与地域分布无关,来源于不同宿主的菌株的16SrDNA变异并不大,而与E.faecium、E.canis、E.avium、E.hirae等...

In this study,Gram＇s staining of positive and catalase negative coccus strains（n= 4Z） was isolated from clinleal diseased pig in Hunan province, most of those strains were from lung（78.6%）. The colonial morphology and the shape of Cram strain bear a close analogy to ATCC29212 strain. Laneefield test showed that 97.6%（41/42）strains belongs to group D. The sequence analysis of 16S rDNA revealed that all Hunan strains share a high identity with reference strain ATCC29212（99.2%）and the homology ranged from 98.7 ~ to 100 ~ when comparing wi...