期刊论文

中文

中国兽医学报

1005-4545

2012

32

10

1440-1443,1472

10.16303/j.cnki.1005-4545.2012.10.002

国家自然科学基金资助项目（30972167）；

本校为第一机构

为探讨猪圆环病毒2型（PCV2）对小鼠超微结构的病理损伤及其病毒在细胞内的复制,20只6周龄的昆明小鼠随机平均分成2组（即A组和B组）,A组小鼠经腹腔注射PCV2细胞培养物0.1mL/只（含病毒1 000TCID50）,B组以同样的方式和剂量注射无菌细胞培养液作为对照。于PCV2感染后14d,处死所有小鼠,取其组织做电镜观察和PCV2PCR检测。结果显示,在电镜下,所有PCV2感染鼠的超微结构病变基本一致,主要表现为淋巴器官、心脏、肝脏、肺脏、肾脏、脑、肠等脏器实质细胞凋亡或坏死,细胞内线粒体水肿、内质网扩张,间质毛细血管淤血、炎症细胞浸润,并在脾脏、胸腺、淋巴结的淋巴细、巨噬细胞,以及肝细胞,肾足细胞,脑神...

In order to explore the ultra-microscopic lesions and viral replication in Kunming mice infected with type 2 of porcine circovirus（PCV2）,20 mice random equally divided into 2 groups,namely group A and group B.Mice in group A were injected intraperitoneally with 0.1 mL of viral cell cultures（containing virus 1 000 TCID50） per mouse,and group B were injected with equal volume of cell cultures without virus as same method.Tissues from all euthanatized mice were collected at 14 dpi for preparation of transmission electron microscopy（TEM） and ...