期刊论文

中文

中国兽医学报

1005-4545

2017

37

11

2101-2107

10.16303/j.cnki.1005-4545.2017.11.12

湖南省教育厅优秀青年基金资助项目（13B050）；

本校为第一机构

将从健康仔猪分离到的猪肺泡巨噬细胞(PAM)分别按1∶10、1∶3和1∶1的比例与猪肺炎支原体(Mhp)232株共同孵育。在孵育12 h后收获PAM,用qPCR检测PAM中MHCⅡ分子mRNA表达量,确定PAM和Mhp的最佳孵育比(ROI)。将PAM和Mhp按ROI比例混合孵育(试验组),用等体积的10% RPMI-1640液代替Mhp与PAM孵育作为对照组。分别在孵育后12,24,36,48 h收获试验组和对照组的PAM。用实时荧光定量PCR(qPCR)检测PAM中与外源性抗原加工递呈功能相关分子MHCⅡ、SLA-DM、Li和CD86、TLR2和TLR6 mRNA表达量,分析Mhp感染对PAM外源性杭原递呈功能的影响。结果显示:相关因子MHCⅡ、SLA-D、Li、CD86和TLR6在Mhp感染PAM 12 h后,mRNA表...

The PAM isolated from healthy piglets,were incubated as the proportion of 1： 10,1： 3 and 1： 1 with Mycoplasma hyopneurnoniae （Mhp） strain 232. After incubation 12 h,PAM was harvested and detected by qPCR methods to determine the relative transcription of MHCⅡ mR- NA,and the optimal incubation ratio （ROD of MHCⅡ and Mhp. PAM and Mhp were incubated by the ROI,and the 10% RPMI-1640 solution,instead of Mhp,was incubated with PAM as the control group. PAM were harvested at 12 h post incubation （i2 hpi）,24,36 and 48 hpi,respectively. qPCRs ...