期刊论文

中文

中国兽医学报

1005-4545

2017

37

8

1553-1557

10.16303/j.cnki.1005-4545.2017.08.22

国家自然科学基金资助项目（31572563） 湖南省研究生科研创新资助项目（CX20148285）

本校为第一机构

为揭示钩吻素子的体外抗炎作用机理,利用硝酸还原酶法和ELISA法研究了不同质量浓度(100,200,400mg/L)的钩吻素子对一氧化氮(NO)和细胞因子白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)分泌的影响,运用了QPCR的方法检测了钩吻素子对诱导型一氧化氮合成酶(iNOS)、IL-1β、IL-6和TNF-αmRNA表达水平的影响,并运用Western blot法检测了钩吻素子对iNOS蛋白表达的影响。结果显示,100,200,400mg/L质量浓度的钩吻素子能够极显著的抑制RAW264.7细胞NO的产生以及IL-1β、IL-6和TNF-α的分泌(P

To reveal the anti-inflammation mechanism of koumine,we determined effects of different concentrations of koumine（100,200,400 mg/L） on the secretion of NO, IL-1β, IL-6 and TNF-a by nitrate reductase and ELISA as well as the mRNA of iNOS, IL-1β,IL-6 and TNF-α. Western blot was used to detect iNOS protein expression. The results showed that 100,200,400 mg/L of koumine can significantly inhibit the secretion of NO,IL-1β, IL-6 and TNF-α of RAW264.7 cell （P〈0.01）. Koumine can dose-dependently down-regulate the mRNA of iNOS, IL-1β, IL-6 an...