期刊论文

The quantification of dihydrosanguinarine and dihydrochelerythrine in chicken muscle

中文

中国兽医学报

1005-4545

2018

38

9

1766-1770

10.16303/j.cnki.1005-4545.2018.09.24

国家重点研发计划基金资助项目（2017YFD0501503）；

本校为第一机构

建立了一种用于同时测定鸡肌肉中二氢血根碱和二氢白屈菜红碱的UPLC-MS/MS方法.肌肉样品经乙腈-水(95∶5)溶液超声提取后,离心取上清液经脱脂后氮吹浓缩,甲醇复溶.采用Agilent Poroshell 120 EC-C18(2.1 mm×150 mm i.d.,2.7 μm)色谱柱分离,采用正离子化模式下的多反应监测模式进行测定.方法的特异性良好,二氢血根碱和二氢白屈菜红碱在0.2～200 μg/L质量浓度范围内线性关系良好(r2≥0.999 6),最低定量限均为0.4 μg/kg,平均回收率分别为103.44％和104.36％,准确度RE均≤10.49％,精密度RSD均≤7.43％.该方法应用于长期饲喂博落回散的健康雌性白羽肉鸡的鸡胸肌组织中二氢血根碱和二氢白屈菜红碱...

A UPLC-MS/MS method has been developed for the quantification of dihydrosanguinarine and dihydrochelerythrine in broiler chickens muscle.The muscle were extracted by acetonitrile-water(95∶5)solution in ultrasonic bath,the supernatant was degreased and dried with nitrogen after centrifugation,then refolded by methanol.The analytes were separated on Agilent Poroshell 120EC-C_(18)column.Mass spectrometric detection was performed by multiple reactions monitoring mode via electrospray ionization source operating in positive ionization mode.The meth...