期刊论文

Research progress of the structure of PCV2Cap Loops and the construction of PCV2 virus-like particles

中文

中国兽医学报

1005-4545

2018

38

1

235-240

10.16303/j.cnki.1005-4545.2018.01.38

湖南省教育厅重点资助项目（15A086） 湖南省科技计划重点资助项目（2014FJ2011）

本校为第一机构

病毒样颗粒(virus-like particles,VLPs)是某种病毒的1种或几种结构蛋白在体外组装而成,由于VLPs不包含病毒基因组,在宿主细胞中不能自我复制,因此VLPs在亚单位疫苗应用中具有较高的生物安全性。自猪圆环病毒2型(porcine circovirus type 2,PCV2)在世界范围内流行以来,已经给全球养猪业带来巨大经济损失,因此PCV2的致病机理和疫苗研究具有重要现实意义。PCV2Cap蛋白(capsid protein)是病毒唯一的衣壳蛋白,也是主要的免疫原性蛋白,60个Cap蛋白亚基在体外能自行组装成PCV2VLPs,该VLPs因与天然病毒粒子形态相似并保留了病毒完整的构象表位,已经在PCV2组装和侵染机理研究、亚单位疫苗的研发及其相关...

Virus-like particles （VLPs） are spontaneously assembled by one or several viral structure proteins in vitro. VLPs have no characteristics of self-replication in host cells due to absence of viral genomes,and have a stronger safety profile in subunit vaccine application. Porcine circovirus type 2（PCV2） is a common virus of pigs found throughout the world,causing huge economic loss for the worldwide swine industry, the pathogenic mechanism and effective vaccines against PCV2 have important significance. As the only structural protein of viral...