期刊论文

In vitro metabolism of koumine in pig

中文

中国兽医学报

1005-4545

2018

38

8

1568-1572

10.16303/j.cnki.1005-4545.2018.08.18

湖南省***基金资助项目（2017JJ1017）；

本校为第一机构

研究钩吻素子在猪体外肝S9的代谢,采用高效液相色谱串联四极杆飞行时间质谱(HPLC/QqTOF MS)对孵育后的代谢物进行结构鉴定。结果表明,钩吻素子在猪肝S9中可生成7种代谢产物,根据原形及其代谢物的精确分子质量差、产物离子,鉴定了代谢物结构,包括N-脱甲基化代谢物(M1)、C18和C19的加氢代谢物(M3)、M3的去甲基代谢物(M4)、氧化代谢物(M6、M8和M12)和脱氢代谢物(M13)。钩吻素子在猪的代谢途径主要是氧化、还原、 N-脱甲基化。其研究结果为钩吻素子在动物及人体内代谢研究奠定基础。

A rapid and accurate high-performance liquid chromatography/quadrupole-time-of-flight （HPLC/QqTOF） mass spectrometry method was applied to characterize koumine metabolites in liver $9 of pig. As a result,a total of seven metabolites of koumine were identified by the comparing their changes in accurate molecular masses and product ions with those of the precursor ion or metabolite,including N-demethylated metabolites （M1）, C18 and C19 hydrogenated metabolites （M3）, N-demethylated-hydrogenated metabolites （M4）, oxidative metabolites （M6,...