期刊论文

Effects of compound immune stress on immune function and liver lipid metabolism in broilers

中文

中国兽医学报

1005-4545

2019

39

09

1794-1800

10.16303/j.cnki.1005-4545.2019.09.22

2017JJ3108:湖南省自然科学基金 2016YFD0501209:国家重点研发计划 2017GK5009:湖南省创新创业技术投资项目

本校为第一机构

旨在评估免疫应激状态下AA肉鸡新城疫疫苗免疫效果,观察其对免疫功能和肝脏脂代谢的影响以及抗应激剂的调控效果.选取160只AA肉仔鸡随机分为A、B、C、D、E等5个组,每组32只.A组为对照组,B、C组为250 μg/kg细菌脂多糖(LPS)处理组,D、E组为500 μg/kg LPS处理组;其中,在C、E组饲料中添加1％的抗应激剂.于12日龄起每隔3d连续腹腔注射LPS共4次,于15,28 d各组分别接种鸡新城疫(ND)Ⅳ系苗,并于22,29,36,42 d将肉鸡称重、宰杀,检测抗体水平、免疫器官指数、肝脏胆固醇(TC)和甘油三酯(TG)含量及其脂代谢相关基因mRNA表达.结果 显示,与A组相比,D组能够显著增加法氏囊、胸腺和脾脏等免疫器官指数(P＜0.05...

The effect of immune stress on immune function,hepatic lipid metabolism,and the regulation of anti-stress agents under vaccination with Newcastle disease vaccine in broilers,was assessed. A total of 160 broilers were randomly assigned to 5 groups:group A served as the control, group B and C received 250μg/kg LPS,group D and E received 500μg/kg LPS,1%anti-stress agent was fed in group C and E.All the groups were vaccinated with live LaSota ND vaccine on 15 and 28 d.The LPS was intraperitoneal injected on 12,15,18 and 21 d,and the chickens were...