期刊论文

Genetic variation analysis of porcine epidemic diarrhea virus isolated from pig farm in Henan Province

中文

中国兽医学报

1005-4545

2020

40

6

1073-1079

10.16303/j.cnki.1005-4545.2020.06.01

“十三·五”国家重点研发计划资助项目(2017YFD0500101)。

本校为第一机构

为分析河南省猪流行性腹泻病毒(PEDV)S基因的遗传变异情况,设计并合成能够扩增N基因和S基因的引物,利用RT-PCR方法对2017年1月至2018年5月河南省11个地区规模猪场的178份病料进行检测、克隆和测序,并在氨基酸水平上将本研究分离株的S基因与国内外流行毒株的S基因进行比对分析.结果 显示,河南省11个地区的178份样品,PEDV阳性样品113份,阳性率63.5％(113/178).选取29株PEDV代表株进行氨基酸序列比较,结果显示,29株分离株与经典株CV777同源性为88.4％～90.3％,与经典株CV777相比,29株代表株在S1区域存在碱基的插入、缺失和变异现象;基于S基因的遗传进化分析表明,本研究的29株分离株均属于G2群,而以...

In order to study the variation of S gene from the porcine epidemic diarrhea virus,the N gene and the S gene primers were designed.The 178 diarrhea samples were detected using reverse transcription-polymerase chain reaction(RT-PCR),cloned and sequenced.These samples were collected from large-scale pig farms in 11 regions of Henan Province in January 2017 to May 2018.The amino acid of S gene was analyzed and compared with the other published PEDV strains.The results indicated that 113 samples in 11 regions showed PEDV positive,and the positive rate was 63.5%(113/178).A total of 29 PEDV represen...