[Zhao, Jianhua; Tian, Xudan] Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China.
通讯机构：
[Liangying Dai] C
[Shaohong Qu] I
College of Plant Protection, Hunan Agricultural University, Changsha, China<&wdkj&>Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
This work was financially supported by the State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products in Zhejiang Academy of Agricultural Sciences, and by the National Natural Science Foundation of China (Grant No. 31672016).
机构署名：
本校为第一机构
院系归属：
植物保护学院
摘要：
BACKGROUND: Biotechnologists seeking to develop marker-free transgenic plants have established co-transformation methods. For co-transformation using mixed Agrobacterium strains, the mix ratio of Agrobacterium strains and selection scheme may influence co-transformation frequency. This study used fluorescent GFP and RFP markers to compose different selection schemes for observation of the selective dynamics of transformed rice cells and to investigate the factors affecting co-transformation efficiency. METHODS AND RESULTS: We utilized GFP and RFP markers in co-transformation and tested the com...
