To explore the genetic relationship of 42 strains of Enterococcus faecalis (from swine) isolated from different regions of Hunan Province, 42 strains were classified by using amplified fragment length polymorphism (AFLP) method. The results showed that strains had diversity in the gene, according to similarity of 75%, 21 genotypes can be classified; CS64, CS81 and CS86 had a close genetic relation and obtained AFLP map and virulence genotypes of a special correlation. This test applied AFLP to lyze the genetic differences, combined with the...