期刊论文

Determination of Quercetin Content in Raw Drug by Ultraviolet Spectrophotometry

中文

动物医学进展

1007-5038

2016

37

1

122-124

10.16437/j.cnki.1007-5038.2016.01.028

(CX2015B257):湖南省研究生科研创新项目 :湖南农业大学大学生科技创新基金

本校为第一机构

为构建一种简便、高效的槲皮素原料药含量的测定方法,采用紫外分光光度法对槲皮素原料药进行含量测定,并进行方法学考察.结果表明,紫外分光光度法在374 nm处测定的槲皮素原料药平均含量为97.97％,标准曲线方程为y=0.007 4x-0.006 7,R2 =0.999 7,在20.8 μg～140 μg范围内有良好的线性关系,平均加样回收率为100.88％,RSD为0.96％(n=6),其精密度较好,符合方法学要求.说明该法适用于槲皮素原料药含量测定,是一种经济、简便与高效的质量分析方法.

In order to build a simple and efficient method to assay the quercetin content in raw,the ultraviolet spectrophotometry was used and its methodology is studied.The results showed that the average content of quercetin is 97.97%,and the standard curve and regression equation that is y=0.007 4x-0.006 7（r=0.999 7）were established by the ultraviolet spectrophotometry.There is a good linear relationship between 20.8μg to 140μg.And the average recovery of the method is 100.88%,and RSD is 0.96%（n=6）.The results of methodological study showed that...