Peripartum period is a time when common diseases and contagious diseases of ruminants often take place,and its most prominent pathological feature is the negative energy balance (NEB).The liver is the major organ for energy metabolism, and thus the collection of liver samples is of particularly importance for the diagnosis and study of pathogenesis of perinatal diseases.In the present study, needle biopsy was employed for liver tissue sampling in peripartum goats.Standard operation of needle puncture was applied to 9 peripartum goats,and 70 t...