期刊论文

Establishment of LAMP Method for Detection of nuc Gene of Staphylococcus aureus from Dairy Cow Mastitis

中文

动物医学进展

1007-5038

2023

44

08

34-38

10.3969/j.issn.1007-5038.2023.08.008

2022NK2025:湖南省重点研发计划项目 kh2201218:长沙市科技计划项目

本校为第一机构

为了快速鉴定因金黄色葡萄球菌感染引起的奶牛乳房炎,采用环介导等温扩增(LAMP)技术和传统PCR技术,针对金黄色葡萄球菌nuc基因设计特异性引物并进行扩增鉴定,同时对两种鉴定方法的最适反应条件、灵敏度和特异性进行了研究。...展开更多 为了快速鉴定因金黄色葡萄球菌感染引起的奶牛乳房炎,采用环介导等温扩增(LAMP)技术和传统PCR技术,针对金黄色葡萄球菌nuc基因设计特异性引物并进行扩增鉴定,同时对两种鉴定方法的最适反应条件、灵敏度和特异性进行了研究。结果显示,LAMP和传统PCR均能成功扩增金黄色葡萄球菌的nuc基因,但通过优化LAMP反应条件,在62℃时对nuc基因呈现最佳扩增效果,与传统PCR鉴定...

In order to quickly identify dairy cow mastitis caused by Staphylococcus aureus infection,loop-mediated isothermal amplification(LAMP)and traditional PCR were used in this study.Specific primers were designed and amplified to identify the nuc gene of Staphylococcus aure...MORE In order to quickly identify dairy cow mastitis caused by Staphylococcus aureus infection,loop-mediated isothermal amplification(LAMP)and traditional PCR were used in this study.Specific primers were designed and amplified to identify the nuc gene of Staphylococcus aureus...