Engineering of rice varieties with enhanced resistances to both blast and bacterial blight diseases via CRISPR/Cas9

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期刊论文

作者：

Zhou, Yanbiao*;Xu, Shichong;Jiang, Nan;Zhao, Xinhui;Bai, Zhenan;...

通讯作者：

Zhou, Yanbiao;Yang, Yuanzhu;Tan, Junjie

作者机构：

[Lv, Chao; Tang, Qianying; Jiang, Nan; Hu, Xiaochun; Zhou, Yanbiao; Yang, Yuanzhu; Bai, Zhenan; Zhao, Xinhui; Wang, Kai; Xu, Shichong] Yuan Longping High Tech Agr Co Ltd, Minist Agr & Rural Affairs, Hunan Engn Lab Dis & Pest Resistant Rice Breeding, Key Lab Southern Rice Innovat & Improvement, Changsha 410001, Hunan, Peoples R China.

[Zhou, Yanbiao] South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China.

[Lv, Chao; Yang, Yuanzhu; Xu, Shichong] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China.

[Liu, Jinling; Yao, Wei; Zhao, Xinhui; Yang, Yuanzhu] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.

[Xiao, Gui; Yang, Yuanzhu] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.

通讯机构：

[Zhou, YBA; Yang, YZ] Y

[Zhou, Yanbiao] S

[Yang, Yuanzhu] H

[Tan, JJ; Yang, YZ] N

Yuan Longping High Tech Agr Co Ltd, Minist Agr & Rural Affairs, Hunan Engn Lab Dis & Pest Resistant Rice Breeding, Key Lab Southern Rice Innovat & Improvement, Changsha 410001, Hunan, Peoples R China.

语种：

英文

关键词：

CRISPR/Cas9;TGMS;bacterial blight;rice;rice blast

期刊：

Plant Biotechnology Journal

ISSN：

1467-7644

年：

2022

卷：

期：

页码：

876-885

DOI：

10.1111/pbi.13766

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31901516]; special funds for the construction of innovative provinces in Hunan [2020RC3085]; Project of Hunan Natural Science Youth Foundation [2019JJ50414]

机构署名：

本校为通讯机构

院系归属：

农学院

摘要：

Rice blast and bacterial blight represent two of major diseases having devastating impact on the yield of rice in most rice-growing countries. Developments of resistant cultivars are the most economic and effective strategy to control these diseases. Here, we used CRISPR/Cas9-mediated gene editing to rapidly install mutations in three known broad-spectrum blast-resistant genes, Bsr-d1, Pi21 and ERF922, in an indica thermosensitive genic male sterile (TGMS) rice line Longke638S (LK638S). We obtained transgene-free homozygous single or triple mut...

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Engineering of rice varieties with enhanced resistances to both blast and bacterial blight diseases via CRISPR/Cas9

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