Development and evaluation of an entirely solution-based combinative sample preparation method for membrane proteomics

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成果类型：

期刊论文

作者：

Lin, Yong;Liu, Hui;Liu, Zhonghua;Liu, Yi;He, Quanze;...

通讯作者：

Wang, Xianchun

作者机构：

[Wang, Xianchun; Chen, Ping; He, Quanze; Liu, Yi; Lin, Yong; Liang, Songping; Liu, Hui] Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, Minist Educ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

[Liu, Zhonghua; Lin, Yong] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China.

[Liu, Zhonghua; Lin, Yong] Hunan Agr Univ, Key Lab Crop Germplasm Innovat & Utilizat Hunan P, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Wang, Xianchun] H

Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, Minist Educ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

语种：

英文

关键词：

Combinative method;LC-MS/MS;Membrane proteome;Sample preparation;Shotgun analysis

期刊：

Analytical Biochemistry

ISSN：

0003-2697

年：

2013

卷：

432

期：

页码：

41-48

DOI：

10.1016/j.ab.2012.09.023

基金类别：

Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [11JJ2019]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31070700, 31100502]; Specialized Research Foundation for the Doctoral Program of Higher Education of ChinaSpecialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20094306110003, 20114320120004]; National Basic Research Program or "973 Program" of ChinaNational Basic Research Program of China [2010CB529800]

机构署名：

本校为其他机构

院系归属：

园艺园林学院

摘要：

The hydrophobic nature of many membrane proteins, especially integral membrane proteins, brings great difficulties to their analysis. To improve the analysis of membrane proteins, an entirely solution-based combinative sample preparation (CSP) method was developed and its application to the shotgun analysis of rat liver membrane proteomes was evaluated in this study. This CSP method comprehensively uses the strong ability of sodium dodecyl sulfate (SDS) to lyse the membranes and solubilize hydrophobic membrane proteins, the high efficiency of t...

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Development and evaluation of an entirely solution-based combinative sample preparation method for membrane proteomics

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