期刊论文

Xia, B

英文

Bioresource Technology

0960-8524

2024

412

131401

10.1016/j.biortech.2024.131401

CRediT authorship contribution statement Xiao Chen: Writing – review & editing, Writing – original draft, Software, Methodology, Investigation, Data curation, Conceptualization. Li Pang: Writing – review & editing, Software, Methodology, Investigation, Data curation. Wentao Yang: Writing – review & editing. Hong Tian: Writing – review & editing. Youjin Yi: Writing – review & editing, Conceptualization. Bo Xia: Writing – review & editing, Supervision, Project administration, acquisition, Conceptualization.

本校为第一且通讯机构

N-acetyl-D-glucosamine and its dimer are degradation products of chitin waste with great potential in therapeutic and agricultural applications. However, the hydrolysis of insoluble chitin by chitinases remains a major bottleneck. This study investigated the biochemical properties and catalytic mechanisms of PoChi chitinase obtained from Penicillium oxalicum with a focus on enhancing its efficiency during the degradation of insoluble chitin. Recombinant plasmids were engineered to incorporate chitin-binding (ChBD) and/or fibronectin III (FnIII) domains. Notably, PoChi-FnIII-ChBD exhibited the ...