Objective: To determine the volatile oil of the leaves from Hypericum perforation L. . Method: The essential oil was obtained by simultaneous distillation and solvent extraction. The chemical components were separated and identified by GC-MS. The relative contents in the volatile oil were determined by area nomalization. Results: 54 chemical constituents were separated and identified representing 92.32% of the total contents. Conclusion: Sesquiterpenes are major chemical constituents in the oil from the leaves of Hypericum perforatum L. . They are very different from th...