期刊论文

Tan, P.X.

中文

中药材

1001-4454

2011

34

10

1575-1579

10.13863/j.issn1001-4454.2011.10.012

科技部国际合作项目（中日政府间科技合作专项）（2009DFA32350）；

本校为第一机构

目的:探讨黄连提取物对耐药金黄色葡萄球菌的耐药抑制机理。方法:采用荧光分光光度法、SDS-PAGE电泳法和半自动生化仪器分别测定了黄连提取物作用前后耐药性金黄色葡萄球菌体内的药物蓄积量、细菌蛋白电泳图谱及细菌胞外某些酶活性。结果:黄连提取物处理后菌体内诺氟沙星蓄积浓度显著高于空白对照组(P0.05)。结论:黄连提取物对耐药金葡菌的耐药抑制作用是多种化学成分、多靶位共同作用的结果,但确切的分子水平机理还有待进一步的深入研究。

OBJECTIVE: To discuss the inhibit mechanism primarily of extracts from Coptis on clinical resistant the medicine of Staphylococcus aureus. METHODS: Used SDS-PAGE electrophoresis, spectrophotometry and semiautomatic biochemistry analysis to detect the accumulated concentration of norfloxacin, membranin electrophoretogram and the enzymatic activity in extracellular fluid before and after Coptis extract disposition. RESULTS: The accumulated concentration of medicine norfloxacin within the experiment strains which treated with Coptis extract was clear higher than that of the blank space (P < 0.01)...