The liquid of the 15 th flammulina velutipes of culture term was studied. After the single factor experiments the suitable feat carbon souree, nitrogen souree , culture temperatures, the most suitable pH, the deal of the liquid and the inoculating deal would be decided. And after the cross experiment the most suitable culture environment would be obtained : corn powder 5%, potato powder 2%, KH2PO4 0.1%, MBSO4·7H2O 0.05%, VB 110 μg/100 mL, VB250 μg/100 mL. And the suitable, shaking-culture eondition would be : culture temperature as 25℃ , ...