期刊论文

中文

亚热带植物科学

1009-7791

2008

37

3

10-14

10.3969/j.issn.1009-7791.2008.03.003

湖南省自然科学基金项目（06JJ2087）； 湖南农业大学人才基金项目（06WD08）资助；

本校为第一机构

利用RAPD分子标记技术对48个烟草品种进行遗传多样性研究。从200个10bp的随机引物用RAPD方法筛选获得28个多态性引物，然后对48份烟草种质资源的基因组DNA进行扩增，共获得184条DNA扩增片断带。其中多态性带86条，平均多态检出率为46.7％。48份材料的遗传距离为0～7．81，采用UPGMA法聚类分析，可将其分为两大类群，即黄花烟草群与普通烟草群，后者又可分为4组。

RAPD molecular-marker technique was used to detect the genetic diversity among 48 tobacco germplasms. The genomic DNA of 48 tobacco germplasms was tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 arbitrary 10-mer primers. The results showed that the 184 RAPD bands were obtained altogether with 86 bands of polymorphic ones. The percentage of polymorphic bands was 46.7%. The variation range of genetic distances was 0 to 7.81. These varieties were divided into N. tobacum group and N. rustica group by UPGMA cluster analysis based on RAPD molecu...