期刊论文

中文

亚热带植物科学

1009-7791

2008

37

3

43-45

10.3969/j.issn.1009-7791.2008.03.011

湖南省长沙市科技计划重大专项（K051003-22）资助

本校为第一机构

对蝴蝶兰丛生芽、原球茎途径的组织培养进行研究，结果表明，不经过愈伤组织直接诱导丛生芽的过程中，1／2MS＋6-BA6．0mg／L和1／2MS＋6-BA3．0mg／L＋NAA0.2mg／L两种培养基的诱导率可达100％，丛生芽增殖阶段采用1／2MS＋6-BA5．0mg／L＋NAA0.5mg／L效果最好；原球茎途径中，1／2MS＋6-BA15．0mg／L＋NAA1．0mg／L诱导率达20.0％，接入1／2MS＋6-BA3．0mg／L＋NAA0．5mg／L培养基中，增殖倍数达7．4，随后转入1／2MS＋6-BA1.0mg／L＋NAA0．5mg／L中，可很快分化成芽。生根培养基以1／2MS＋IBA0．3mg／L较好，将生根的蝴蝶兰移栽至水苔中，两个月后成活率达100％。此外，蝴蝶兰移栽6个月后转至...

The experiment studied in the tissue culture of Phalaenopsis spp.. The results showed that in 1/2MS medium with 6-BA3.0mg/L＋NAA0.2mg/L or with 6-BA 6.0mg/L, the induction rate of clustered shoots reached 100%. During the proliferating period of clustered shoots, the 1/2MS medium with 6-BA5.0mg/L＋NAA0.5mg/L showed the best effects; in 1/2MS medium with 6-BA 15.0mg/L ＋ NAA 1.0mg/L, the induction rate of protocorm-like body reached 20%, and in 1/2MS medium with 6-BA3.0mg/L＋NAA0.5mg/L, the coefficient of reproduction is up to 7.4. It was easy f...