期刊论文

Study on Protoplasts Isolation of Arachis hypogaea

中文

亚热带植物科学

1009-7791

2023

52

03

220-227

10.3969/j.issn.1009-7791.2023.03.007

32261143733:国家自然科学基金 2022JJ30294:湖南省自然科学基金项目

本校为第一机构

以花生多粒型四粒红Arachis hypogaea var. fastigiate ‘Silihong’为材料,调节不同酶的浓度,优化原生质体分离条件。结果显示,混合酶解液的分离效果显著优于崩溃酶。制备花生60 d龄植株...展开更多 以花生多粒型四粒红Arachis hypogaea var. fastigiate ‘Silihong’为材料,调节不同酶的浓度,优化原生质体分离条件。结果显示,混合酶解液的分离效果显著优于崩溃酶。制备花生60 d龄植株叶片原生质体的适宜条件为:2%纤维素酶+3%果胶酶+1%离析酶+0.02 mol·L-1 KCl+0.02 mol·L-1 MES+0.01 mol·L-1 CaCl2+0.6 mol·L-1甘露醇+0.1%BSA,28℃黑暗酶解3 h。在此条件下,花生叶片原生质体产量为2.965×...

Using leaves and petal of Arachis hypogaea var.fastigiate‘Silihong’as materials,protoplast isolation conditions were optimized through modifying the concentrations of different enzymes.The results showed that the protoplast outcome by using the mixed enzymes was signifi...MORE Using leaves and petal of Arachis hypogaea var.fastigiate‘Silihong’as materials,protoplast isolation conditions were optimized through modifying the concentrations of different enzymes.The results showed that the protoplast outcome by using the mixed enzymes was signi...