期刊论文

Zhao, Qian(zhaoqian@hunau.edu.cn);Shi, Xingbo(shixingbo123@aliyun.com)

英文

ACS Applied Materials & Interfaces

1944-8244

2021

13

22

25738-25747

10.1021/acsami.1c04751

National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31972155, 32001778]; Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2019JJ40115, 2020JJ5249, 2020RC3050]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2018M642980]; Training Program of Excellent Young Innovators of Changsha [kq1905017, kq2009058]

本校为第一机构

This work aims to develop a novel multimode (photothermal/colorimetric/fluorescent) nanozyme-linked immunosorbent assay (NLISA) based on the in situ generation of Prussian blue nanoparticles (PBNPs) on the surface of magnetic nanoparticles (MNPs). Being considered the most toxic among the mycotoxins, aflatoxin B1 (AFB1) was chosen as the proof-of-concept target. In this strategy, MNPs, on which an AFB1 aptamer was previously assembled via streptavidin-biotin linkage, are anchored to 96-well plates by AFB1 and antibody. In the presence of HCl an...