期刊论文

Liu, Y.

中文

土壤学报

0564-3929

2022

59

1

285-296

10.11766/trxb202007140240

* 国家自然科学基金项目（41771300，41301274）和国家重点研发计划项目（2017YFD0800504）资助 Supported by the National Natural Science Foundation of China（Nos. 41771300 and 41301274）and the National Key Research and Development Program of China（No. 2017YFD0800504）

本校为第一机构

长期施肥影响稻田土壤理化性质和硝化微生物群落,但长期施肥对稻田不同土层氨氧化古菌(AOA)和氨氧化细菌(AOB)群落结构的影响尚不明确.以湖南宁乡稻田不同施肥制度长期定位试验为平台,选取不施肥(CK)、施秸秆有机肥(ST)、有机-无机肥配施(OM)和施全量化肥(NPK)4个处理,采用实时荧光定量PCR和Illumina MiSeq测序等技术,系统分析稻田不同土层(0～10、10～20、20～30和30～40 cm)理化性质和AOA、AOB丰度及组成的变化.结果表明,ST、OM和NPK处理显著提高各土层有机碳(SOC)、全氮(TN)、有效磷(Olsen-P)的含量及硝化势(NP),降低土壤pH.各处理中SOC、TN、碱解氮(AHN)和Olsen-P的含量及NP随土壤深度的增加...

[Objective]Nitrification is a critical process of the nitrogen cycle which is driven by ammonia-oxidizing archaea (AOA) and bacteria (AOB). It has been observed that long-term fertilization affect soil properties and nitrifying microbial community in paddy soils. However, the effect of long-term fertilization on the community structure of AOA and AOB along the depth in paddy soils has been given little attention. [Method]From a long-term experiment on fertilization management of a paddy field in Ningxiang, Hunan Province, we selected four treat...