Development and validation of universal PCR, basic ERA, and qPCR assays targeting the 16S-23S rRNA intergenic spacer region for Mycoplasma detection

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Yin, Ruiru;Gan, Lanxi;Li, Shiyang;Zhao, Han;Wei, Yanna;...

通讯作者：

Feng, Zhixin;Yu, YF

作者机构：

[Feng, Zhixin; Yin, Ruiru; Feng, ZX; Yu, Yanfei; Yu, YF] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China.

[Feng, Zhixin; Yin, Ruiru; Gan, Lanxi; Yu, Yanfei; Liu, Beibei; Wei, Yanna; Wang, Jia; Li, Shiyang] Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr & Rural Affairs, Nanjing 210014, Peoples R China.

[Gan, Lanxi; Li, Shiyang] Hunan Agr Univ, Coll Vet Med, Changsha 410125, Peoples R China.

[Ning, Lihua; Zhao, Han] Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Nanjing 210014, Peoples R China.

[Yu, Yanfei; Wang, Jia] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Peoples R China.

通讯机构：

[Feng, ZX; Yu, YF ] N

Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China.

语种：

英文

关键词：

ERA;Mycoplasma contamination;PCR;qPCR

期刊：

Microbial Pathogenesis

ISSN：

0882-4010

年：

2025

卷：

205

页码：

107669

DOI：

10.1016/j.micpath.2025.107669

基金类别：

National Natural Science Foundation of China [32172860, 32102675]; Jiangsu Association for Science and Technology Youth Talent Support Program [JSTJ-2023-XH031]; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture Open Fund [SYKF(23)005]

机构署名：

本校为其他机构

院系归属：

动物医学院

摘要：

Mycoplasma contamination poses a persistent challenge in cell culture systems and the production of cell-derived biological products, including vaccines and therapeutic drugs. Current detection methods suffer from several limitations: they risk false-negative results due to incomplete species coverage, exhibit high detection limits in molecular assays, and prove time-consuming while lacking sensitivity for certain fastidious mycoplasma species that grow poorly in vitro , as outlined in pharmacopeial testing standards. To address these issues, w...

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Development and validation of universal PCR, basic ERA, and qPCR assays targeting the 16S-23S rRNA intergenic spacer region for Mycoplasma detection

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