Development and validation of universal PCR, basic ERA, and qPCR assays targeting the 16S-23S rRNA intergenic spacer region for Mycoplasma detection

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Yin, Ruiru;Gan, Lanxi;Li, Shiyang;Zhao, Han;Wei, Yanna;...

通讯作者：

Zhixin Feng

作者机构：

[Feng, Zhixin; Yin, Ruiru; Yu, Yanfei] College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China

[Feng, Zhixin; Yin, Ruiru; Yu, Yanfei] Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, Nanjing, 210014, China

[Gan, Lanxi; Liu, Beibei; Wei, Yanna; Wang, Jia; Li, Shiyang] Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, Nanjing, 210014, China

[Gan, Lanxi; Li, Shiyang] College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410125, China

[Ning, Lihua; Zhao, Han] Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

通讯机构：

[Zhixin Feng] C

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China<&wdkj&>Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China

语种：

英文

关键词：

ERA;Mycoplasma contamination;PCR;qPCR

期刊：

Microbial Pathogenesis

ISSN：

0882-4010

年：

2025

卷：

205

页码：

107669

DOI：

10.1016/j.micpath.2025.107669

机构署名：

本校为其他机构

院系归属：

动物医学院

摘要：

Mycoplasma contamination poses a persistent challenge in cell culture systems and the production of cell-derived biological products, including vaccines and therapeutic drugs. Current detection methods suffer from several limitations: they risk false-negative results due to incomplete species coverage, exhibit high detection limits in molecular assays, and prove time-consuming while lacking sensitivity for certain fastidious mycoplasma species that grow poorly in vitro , as outlined in pharmacopeial testing standards. To address these issues, w...

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Development and validation of universal PCR, basic ERA, and qPCR assays targeting the 16S-23S rRNA intergenic spacer region for Mycoplasma detection

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