An efficient virus-induced gene silencing (VIGS) system for gene functional studies in Miscanthus

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成果类型：

期刊论文

作者：

He, Guo;Zhao, Xuhong;Xu, Yan;Wang, Yu;Zhang, Zhihai;...

通讯作者：

Hu, Ruibo(hurb@qibebt.ac.cn);Li, Shengjun(li_sj@qibebt.ac.cn)

作者机构：

[Xu, Yan; Hu, Ruibo; Li, Shengjun; Wang, Yu; He, Guo; Zhao, Xuhong] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Energy Inst, CAS Key Lab Biofuels,Shandong Prov Key Lab Energy, Qingdao 266101, Peoples R China.

[He, Guo] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.

[Zhang, Zhihai; Hudson, Matthew] Univ Illinois, Carl R Woese Inst Genom Biol, Ctr Adv Bioenergy & Bioprod Innovat, Urbana, IL 61801 USA.

[Xiao, Liang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.

通讯机构：

[Ruibo Hu; Shengjun Li; Ruibo Hu Ruibo Hu Ruibo Hu; Shengjun Li Shengjun Li Shengjun Li] C

CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Shandong Energy Institute, Qingdao New Energy Shandong Laboratory, Qingdao, 266101 People's Republic of China

语种：

英文

关键词：

miscanthus;phytoene desaturase (PDS);sprout infiltration;tobacco rattle virus (TRV);transient gene silencing;virus-induced gene silencing (VIGS)

期刊：

GCB Bioenergy

ISSN：

1757-1693

年：

2023

卷：

期：

页码：

805-820

DOI：

10.1111/gcbb.13051

基金类别：

DOE Center for Advanced Bioenergy and Bioproducts Innovation [DE-SC0018420]; Innovation Fund of Shandong Energy Institute [SEI I202119, SEI I202130]; Joint Funds of the National Natural Science Foundation of China [U1432126]; National Natural Science Foundation of China [31970322]

机构署名：

本校为其他机构

院系归属：

生物科学技术学院

摘要：

Virus-induced gene silencing (VIGS) is a powerful tool for transient gene functional analysis in plants, especially for monocot species (e.g., grasses) that are recalcitrant to transformation. Despite various VIGS systems that have been developed in different plant species, none was previously available for the bioenergy crop Miscanthus. Here, we report the establishment of an efficient and robust VIGS system mediated by Tobacco Rattle Virus (TRV) in Miscanthus. We first investigated the impact of various factors that may affect gene silencing ...

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An efficient virus-induced gene silencing (VIGS) system for gene functional studies in Miscanthus

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