期刊论文

Liu, Meiling

英文

Biosensors and Bioelectronics

0956-5663

2017

92

457-464

10.1016/j.bios.2016.10.080

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21305042, 21375037, 21475043]; Scientific Research Fund of Hunan Provincial Education DepartmentHunan Provincial Education Department [14B116]; Science and Technology DepartmentScience and Technology Development Fund (STDF)United States Agency for International Development (USAID)US-Egypt Science and Technology Joint Fund [14JJ4030, 13JJ2020]; Opening Fund of State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University [2013017]; Collaborative Innovation Center of New Chemical Technologies for Environmental Benignity and Efficient Resource Utilization; Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province

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In this work, a novel immunosensor was constructed based on the alkaline phosphatase (ALP) in situ generating an electroactive substance by enzymatic hydrolysis the inactive substrates. The new signal-amplified strategy for sensitive detection of HIgG was based on the catalytic oxidation of ALP-generated products, ascorbic acid (AA), using carbon-based Ag-Au bimetallic as the catalyst and signal enhancer. Through a sandwich reaction, ALP-Ab2 bioconjugates were captured on the electrode surface and the amplified signal can be obtained as follows...