Development of an indirect competitive enzyme linked immunosorbent assay for the quantitative detection of Mycoplasma hyopneumoniae during the vaccine production process

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成果类型：

期刊论文

作者：

Wei, Yanna;Khoza, Thandeka;Yu, Yanfei;Wang, Li;Liu, Beibei;...

通讯作者：

Feng, Zhixin;Xiong, QY

作者机构：

[Wei, Yanna] Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing, Peoples R China.

[Wang, Li; Yu, Yanfei; Xiong, Qiyan; Liu, Beibei; Wang, Jia; Feng, Zhixin; Xiong, QY; Gan, Lanxi; Hao, Fei; Feng, ZX; Shao, Guoqing; Wei, Yanna] Jiangsu Acad Agr Sci, Inst Vet Med, Minist Agr & Rural Affairs, Key Lab Vet Biol Engn & Technol, Nanjing, Peoples R China.

[Khoza, Thandeka; Wei, Yanna] Univ KwaZulu Natal, Coll Agr Engn & Sci, Discipline Biochem, Sch Life Sci, Private Bag X54001, Durban, South Africa.

[Xiong, Qiyan; Wang, Jia; Shao, Guoqing] Univ KwaZulu Natal, Coll Agr Engn & Sci, Discipline Microbiol, Sch Life Sci, Private Bag X54001, Durban, South Africa.

[Yu, Yanfei; Gan, Lanxi] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.

通讯机构：

[Xiong, QY ; Feng, ZX] J

Jiangsu Acad Agr Sci, Inst Vet Med, Minist Agr & Rural Affairs, Key Lab Vet Biol Engn & Technol, Nanjing, Peoples R China.

语种：

英文

关键词：

*CCU;*Indirect competitive-ELISA;*Mycoplasma hyopneumoniae;*Quantitative detection;*Vaccine

期刊：

Journal of Immunological Methods

ISSN：

0022-1759

年：

2022

卷：

500

页码：

113196

DOI：

10.1016/j.jim.2021.113196

基金类别：

Independent Research Project Program of Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, China [2019sy001]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770193, 31700158, 32172860, 32102675]; 333 High-level Personnel Training Project of Jiangsu Province of China [BRA2020370]; Six Talent Peaks Project in Jiangsu Province of China [NY-015]; Jiangsu Agricultural Science and Technology Innovation Fund [CX(20)3090]; [CX (20) 3090]

机构署名：

本校为其他机构

院系归属：

动物医学院

摘要：

Inactivated Mycoplasma hyopneumoniae vaccine is used extensively to control M. hyopneumoniae infection worldwide. Quantification techniques are essential in the process of standardizing and validating vaccines. In this study, we developed and optimized an indirect competitive enzyme linked immunosorbent assay (ic-ELISA) for the rapid quantification of M. hyopneumoniae antigen during vaccine production. Briefly, whole M. hyopneumoniae antigen was coated onto microtiter plates, and a polyclonal antibody against M. hyopneumoniae recombinant elongation factor thermo unstable (EF-Tu) protein was pr...

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Development of an indirect competitive enzyme linked immunosorbent assay for the quantitative detection of Mycoplasma hyopneumoniae during the vaccine production process

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