期刊论文

中文

作物学报

0496-3490

2006

32

11

1767-1771

10.3321/j.issn:0496-3490.2006.11.030

国家烟草专卖局基金资助项目（110200001012,11020041012）； 湖南农业大学校青年基金；

本校为第一机构

分别在烤烟K326(Nicotiana tabacum)中部第11叶的未熟、适熟、过熟时期,打孔取样.采用鲜样徒手切片观察不同成熟度叶片的解剖结构,并用酶解法游离出单个叶肉细胞,及撕取下表皮观察气孔保卫细胞,测定其中叶绿体数量和叶绿素含量.研究了烤烟成熟过程中叶片组织、细胞结构及叶绿体在形态和数量上的变化规律.发现随着成熟度的增加叶片解剖结构逐渐疏松,细胞轮廓逐渐模糊,叶肉细胞内叶绿体数量逐渐减少,淀粉粒数量逐渐增加,气孔器保卫细胞中叶绿体的数量也呈下降的趋势,单位质量(克)叶片中提取的叶绿体数量和叶绿素含量也有相同的变化趋势.

Flue-cured Tobacco K326（ Nicotiana tabacum） was cultured in the experimental station of Hunan Agricultural University. The leaves were sampled when the middle leaf （the 11^th leaf） was green, ripe and aged. The leaves were sliced up by hand and observed. In order to get single cell, the leaves were treated with cellulase and pectinase. Epidermis was removed from the abaxial surface of a leaf, and the number of chloroplasts in the guard cells was counted. All the observations above were receded with a camera under the optical microscope. The...