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六个甘蓝型油菜油酸脱氢酶(FAD2)假基因的克隆和分析

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成果类型:
期刊论文
作者:
肖钢;张振乾;邬贤梦;谭太龙;官春云
通讯作者:
Guan, C.-Y.
作者机构:
[Zhen-Qian ZHANG; Xian-Meng WU; Chun-Yun GUAN] Institute of Oil Crops/ National Oil Crops Improvement Center, Changsha 410128, China
Pre-State Key Laboratory for Germplasm Innovation and Resource Utilization of Crops, Changsha 410128, China
[Gang XIAO; Tai-Long TAN] Institute of Oil Crops/ National Oil Crops Improvement Center, Changsha 410128, China<&wdkj&>Pre-State Key Laboratory for Germplasm Innovation and Resource Utilization of Crops, Changsha 410128, China
通讯机构:
[Guan, C.-Y.] I
Institute of Oil Crops/ National Oil Crops Improvement Center, Changsha 410128, China
语种:
中文
关键词:
甘蓝型油菜;油酸脱氢酶;基因表达;酿酒酵母
关键词(英文):
FAD2
期刊:
Acta Agronomica Sinica
ISSN:
1875-2780
年:
2010
卷:
36
期:
3
页码:
435-441
基金类别:
This study was financially supported by the National Basic Research Program of China (2006-CB101600).
机构署名:
本校为第一机构
院系归属:
农学院
摘要:
以甘蓝型油菜湘油15为材料,采用PCR方法克隆并分析了56个FAD2基因克隆和47个FAD2基因cDNA克隆,从中发现6个新的FAD2拷贝.它们与公布的甘蓝型油菜FAD2基因(AY577313)具有87.0%以上的同源性,没有内含子,在开放阅读框中存在1~12个终止密码子,其中有2个拷贝具有转录功能.将这6个FAD2拷贝在酿酒酵母中进行体内表达实验,通过气相色谱检测脂肪酸组成证明其不具备油酸脱氢酶功能,与对照相比,也没有改变酵母体内脂肪酸组成.南此推测这6个FAD2拷贝为假基因.
摘要(英文):
The phenomenon of multi-copy genes is common in plants. Pseudogene is defined as an inactive gene, which can not synthesize functional proteins but share the similar DNA sequences with normal functional genes. In this study, 56 FAD2 DNA clones and 47 FAD2 seed cDNA clones of Brassica napus cv. Xiangyou 15 were investigated, and six new copies of FAD2 were detected, designated as FAD2P1-6 respectively. This sequence length of six copies ranged from 1 141–1 157 bp and there were no introns in their open reading frames (ORF). These six copies s...

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