期刊论文

中文

作物学报

0496-3490

2011

37

7

1196-1204

10.3724/SP.J.1006.2011.01196

本研究由国家重点基础研究计划（973计划）项目（2006CBl01600）资助.

本校为第一机构

DOF(DNA binding with one finger)转录因子是植物特有的转录因子家族,含有一个独特的富含Cys残基的单锌指DNA结合区域,在植物生长发育中参与多种生物学过程。本研究根据拟南芥AtDof1.7基因(GenBank登录号为 AT1G51700)序列设计含有不同酶切位点的特异性扩增引物,以拟南芥总DNA为模板,扩增AtDof1.7基因片段,将 AtDof1.7基因正向反向分别插入表达载体的相应位置,构建成AtDof1.7基因的RNA干扰载体pADOF1。利用改良的 floral-dip方法将干扰载体pADOF1成功转入野生型拟南芥,经草甘膦抗性筛选和PCR检测获得5株阳性转基因植株。利用RT-PCR技术和气相色谱法分别分析了AtDof1.7基因的表达和种子脂肪酸组...

The DOF（DNA binding with one finger） transcription factors are members of a family of plant-specific transcription factors that have a highly conserved DNA-binding domain,namely Dof domain which contains a single C2C2-type zinc-finger-like motif and specifically recognizes an（A/T）AAAG sequence as the recognition core.It suggests that the Dof transcription factors play diverse roles in specific biological processes in plants.Members of this protein family in plants are found to be involved in the gene regulation of many processes,but the rol...