期刊论文

Xiong, X.-H.

中文

作物学报

0496-3490

2014

40

10

1748-1755

10.3724/SP.J.1006.2014.01748

国家高技术研究发展计划（863计划）项目（2012AA101107-3） 湖南农业大学作物学开发基金项目（ZWKF201303）资助

本校为第一且通讯机构

植物溶血磷脂酸酰基转移酶(lysophospholipid acid actyltransferase, LPAT)是三酰甘油生物合成过程中的一个关键酶,在脂质的合成、种子的发育以及生物膜的流动性等方面有重要作用。本研究采用同源克隆的方法,获得LPAT4基因的2条全长CDS,长度分别为1143 bp和1140 bp。生物信息学分析表明,它们均具有LPLAT_LCLAT1样结构域,同属于 LPLAT 超基因家族,并分别被命名为 BnLPAT4-1和 BnLPAT4-2。时空表达分析表明,它们均为组成型表达基因,其中 BnPAT4-1在叶中的表达量最高,而 BnLPAT4-2在胚中的表达量最高。逆境分析表明, BnLPAT4-1和 BnLPAT4-2在 NaCl、PEG-4000、水渍、6BA 和 ABA 的胁迫下呈现出不同...

Lysophospholipid acid actyltransferase (LPAT) is a pivotal enzyme of triacylglycerol biosynthesis and plays a key role in lipid synthesis, development of plant seeds and bio-membrane fluidity. Two LPAT4 full-length coding sequences (1143 bp and 1140 bp) were obtained by homology-based cloning, designated BnLPAT4-1 and BnLPAT4-2, respectively. Bioinformatics analysis revealed that they share the LPLAT_LCLAT1 like domain and belong to the LPLAT super family. Temporal and spatial expression results showed that BnLPAT4-1 and BnLPAT4-2 are genes of constitutive expression, with the highest expressi...