期刊论文

Xiong, X.-H.

中文

作物学报

0496-3490

2014

40

7

1304-1310

10.3724/SP.J.1006.2014.01304

本研究由国家高技术研究发展计划（863计划）项目（2012AA101107-3）和湖南农业大学作物学开发基金资助项目（zwKF201303）资助.

本校为第一且通讯机构

甘油-3-磷酸酰基转移酶(sn-glycerol-3-phosphate acyltransferase,GPAT)是三酰甘油生物合成的关键酶,催化三酰甘油合成的起始步骤,在植物中参与调节生长发育、脂质的合成和逆境应答等过程。本试验通过RT-PCR 从甘蓝型油菜品种湘油15中克隆得到GPAT6基因的2个拷贝,分别命名为BnGPAT6-1 (GenBank 登录号为KJ000117)和BnGPAT6-2(KC106728)。它们的编码区序列(coding DNA sequence,CDS)均为1506 bp,编码501个氨基酸。预测其蛋白结构N 端含有一个类卤酸脱卤酶(haloacid dehalogenase-like hydrolase,HAD-like)活性结构域,C 端含有一个溶血磷脂酰基转移酶(lysophospholipid acyltransferase,LPLAT)功...

A key enzyme sn-glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial step of TAG biosynthetic pathway, and is involved in many processes including plant growth, development and response to abiotic stresses. In this study, two B. napus GPAT6 homologs were cloned from the leaf of the cultivar xiangyou 15 using RT-PCR, and designated as BnGPAT6-1 and BnGPAT6-2, respectively. The coding DNA sequences (CDS) of two BnGPAT6 genes are 1506 bp in length, encoding two different polypeptides of 501 amino acid residues. The proteins were predicted to consist of a haloacid dehalogenase-like hy...