期刊论文

Guan, C.-Y.

中文

作物学报

0496-3490

2015

41

11

1663-1670

10.3724/SP.J.1006.2015.01663

湖南省科技创新基金项目（CX2013A012）； 湖南省科技重大专项（2014FJ1006）资助；

本校为第一且通讯机构

脂肪酸去饱和酶基因(FAD2)是控制植物体中油酸含量的关键基因,在甘蓝型油菜中有4个FAD2基因的拷贝,分别定位在A1、C1、A5、C5染色体上。本文克隆了1个FAD2拷贝基因, 依据油菜基因组数据库信息, 将其定位到C1染色体上, 命名为BnFAD2-C1, 其开放阅读框为1155 bp。采用RACE (rapid-amplification of cDNA ends)技术获得了175 bp的5' UTR序列和212 bp的3' UTR序列。采用荧光定量PCR技术研究发现, BnFAD2-C1在根、花和角果皮中仅保持本底水平的表达,在种子发育中期呈现高效表达, 具有种子特异性诱导表达的特征。根据甘蓝和油菜基因组数据库信息, 同源克隆到BnFAD2-C1基因的启动子(promoter)和内含子(...

Fatty acid desaturase gene (FAD2) is a key factor in regulating oleic acid content. There are four copies of FAD2 located on chromosomes A1, C1, A5, and C5 in Brassica napus. One copy containing 1155 bp open reading frame was cloned with previous research method and named as BnFAD2-C1, which was location on chromosome C1 based on the genome database information of oleracea and oilseed. The untranslated regions (UTR) of 5' and 3' end with 175 bp and 212 bp length respectively were cloned by RACE (rapid-amplification of cDNA ends) technique. The expression pattern of BnFAD2-C1 gene was identifie...