期刊论文

Guan, C.-Y.

中文

作物学报

0496-3490

2016

42

5

658-666

10.3724/SP.J.1006.2016.00658

(31401419):国家自然科学基金项目 (13B051):湖南省教育厅优秀青年项目 (2011AA10A104, 2012AA101107):国家高技术研究发展计划(863计划)项目 (11KFXM08):湖南省作物种质资源与创新利用重点实验室科学基金开放项目 (31401419):This work was supported by the National Natural Science Foundation of China (13B051):Outstanding youth project of Hunan Provincial Education Department (2011AA10A104, 2012AA101107):the National High Technology Research and Development Program of China (11KFXM08):Science Foundation of Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization in Crop

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磷脂二酰甘油酰基转移酶(phospholipids:diacylglycerol acyltransferase, PDAT1)是植物三酰甘油(triacylglycerol, TAG)合成的关键酶。本文在甘蓝型油菜湘油15号cDNA中克隆到3个PDAT1全长编码序列(coding sequence, CDS),经比对分别定位于A02、A10、C09染色体,分别命名为BnPDAT1-A02、BnPDAT1-A10和BnPDAT1- C09,其序列长分别为1998、2002和2005 bp,各自编码665、666、667个氨基酸。预测BnPDAT1基因编码蛋白定位于细胞质膜,具有典型的PDAT1保守结构域。多序列比对和进化分析表明, BnPDAT1基因编码蛋白与甘蓝、拟南芥、亚麻芥PDAT1蛋白具有较高的同源性。酵母互补实验证实,该基因编码蛋白具有PD...

Phospholipids:diacylglycerol acyltransferase (PDAT1) is a key enzyme in triacylglycerol (TAG) biosynthesis of plants. In this study, three novel PDAT1 coding sequences (CDSs) were isolated from cDNA of Brassica napus L. cv. Xiangyou 15 seeds, which were mapped to the chromosomes A02, A10, and C09, and designated as BnPDAT1-A02, BnPDAT1-A10, and BnPDAT1-C09, respectively. Three BnPDAT1 CDSs were 1998, 2002, and 2005 bp in length and encoded predicted proteins with 665, 666, and 667 amino acid residues, respectively. BnPDAT1 proteins were predicted to be located on the cell membrane and have a t...