期刊论文

Functional Analysis of BnFAD2-C5 Promoter and Intron at Expression Level in Brassica napus

Guan, C.-Y.

中文

作物学报

0496-3490

2016

42

10

1471-1478

10.3724/SP.J.1006.2016.01471

(CX2013A012):湖南省科技创新项目 (2015CB150200):国家重点基础研究发展规划项目 (CX2013A012):This study was supported by the Science and Technology Innovation Project of Hunan Province (2015CB150200):the National Basic Re-search Program of China

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富含油酸的菜籽油具有重要的经济价值,使得高油酸育种及油酸形成机制成为热点。油酸脱氢酶基因(FAD2基因)是控制油酸含量的关键酶基因。本文针对BnFAD2-C5基因展开研究,根据油菜和甘蓝的同源性,克隆了1257 bp启动子序列,利用GUS和GFP作为报告基因分别构建含有不同片段长度的启动子和内含子的缺失载体并转化拟南芥,经GUS染色检测发现–319 ~ –1 bp为该研究中最小启动子;采用Western blot技术分析启动子和内含子不同区域的功能,发现BnFAD2-C5启动子区域–1257 ~ –1020 bp和–319 ~ –1 bp能够诱导报告基因在转基因拟南芥种子发育中期高效表达, BnFAD2-C5内含子具有增强启动子转录水平的功能,该功...

High oleic rapeseed breeding and the formation mechanism of oleic acid have become a central issue after finding the important economic value of rapeseed oil with high oleic acid. The fatty acid dehydrogenase gene (FAD2) is a key enzyme gene to control oleic acid content, but the regulation of FAD2 gene is not well understood. According to the homology between rapeseed and oleracea, the BnFAD2-C5 promoter sequence of 1257 bp was cloned. Promoter and intron of BnFAD2-C5 gene were analyzed using β-glucuronidase (GUS) reporter and green fluoresce...