期刊论文

Fine Mapping and Candidate Gene Analysis of Rice Blast Resistance Gene Pi47

Xiao, Y.-H.

中文

作物学报

0496-3490

2018

44

7

977-987

10.3724/SP.J.1006.2018.00977

本研究由国家重点研发计划项目(2016YFD0101100), 国家自然科学基金项目(31171834)和湖南省科技重大专项( 2015NK1001-1)资助。 This study was supported by the National Key Research and Development Program (2016YFD0101100), the National Natural Science Foundation of China (31171834), and the Science and Technology Major Project of Hunan Province (2015NK1001-2). * 通信作者(Corresponding authors): 肖应辉, E-mail: xiaoyh@hunau.edu.cn, Tel: +86-731-84618076; 刘金灵, E-mail: liujinling@hunau. edu.cn Received(收稿日期): 2017-11-21; Accepted(接受日期): 2018-03-26; Published online(网络出版日期): 2018-04-27. URL: http://kns.cnki.net/kcms/detail/11.1809.S.20180426.1606.002.html

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不断挖掘和克隆抗稻瘟病新基因, 是解析水稻抗病分子遗传机制和培育抗稻瘟病新品种的重要基础.Pi47是笔者从广谱、持久抗稻瘟病湖南地方品种湘资 3150 中鉴定的稻瘟病抗性基因, 前期研究将其初步定位于第 11 染色体标记RM224和RM5926间.本研究利用3个Pi47单基因系与感病亲本CO39杂交F2群体1687个感病单株对Pi47精细定位, 利用6个STS标记对3个单基因系进行背景分析, 采用生物信息学方法进行了候选基因分析.结果表明, Pi47被精细定位于CAPS标记S32与K33间0.24 cM区域的171.2 kb物理区间内, 背景分析将Pi47进一步缩小至SC12和K33间67.8 kb的区间内; 该区间含有8个结构基因, 其中2个编码NBS-LRR抗病...

Continuous mapping and cloning new blast resistance genes provided an important approach for revealing the molecular mechanism of resistance to rice disease and breeding new varieties resistant to rice blast. Previously, the blast resistance gene Pi47 was mapped between SSR markers RM224 and RM2956 on chromosome 11, from a broad-spectrum and durable resistance native cultivar Xiangzi 3150 in Hunan province. In this study, the fine mapping and candidate gene prediction were performed, showing that Pi47 was mapped in an interval of 0.24 cM betwee...