期刊论文

Bioinformatics analysis and response to nitrate-cadmium stress of NRT1.5 and NRT1.8 family genes in Brassica napus

Zhang, Z.-H.

中文

作物学报

0496-3490

2019

45

3

365-380

10.3724/SP.J.1006.2019.84099

本研究由国家重点研发计划项目(2017YFD0200100, 2017YFD0200103)和国家现代农业(油菜)产业技术体系建设专项资助。 This study was supported by the National Key R&D Program of China (2017YFD0200100, 2017YFD0200103) and the China Agriculture Research System. * 通信作者(Corresponding author): 张振华, E-mail: zhzh1468@163.com 第一作者联系方式: E-mail: ghliang1119@163.com Received(收稿日期): 2018-07-18; Accepted(接受日期): 2018-10-08; Published online(网络出版日期): 2018-11-02. URL: http://kns.cnki.net/kcms/detail/11.1809.s.20181031.1457.002.html

本校为第一且通讯机构

植物对硝酸盐的吸收和转运需要硝酸盐转运体(nitrate transporters, NRTs)的协助。在拟南芥中,硝酸盐的长途转运及其在根部和地上部的分配,主要受NRT1 家族的两个成员NRT1.5 和NRT1.8 的协同调控,且两者的表达均受到硝酸盐的强烈诱导。本文以AtNRT1.5 和AtNRT1.8 基因序列为基础序列,采用生物信息学方法鉴定了白菜、甘蓝和甘蓝型油菜中NRT1.5 和NRT1.8 同源基因,并对基因结构和分子特性、基因拷贝数变异、基因染色体分布、系统进化树、蛋白保守序列比对和跨膜结构域、基因响应低氮和镉胁迫的转录组测序以及基因共表达网络进行了分析。结果表明,白菜、甘蓝及甘蓝型油菜中NRT1.5 和NRT1.8 蛋白均含...

The absorption and transportation of nitrate in plants require the assistance of nitrate transporters (NRTs). The expression of two members of the NRT1 family, including NRT1.5 and NRT1.8 genes was strongly induced by nitrate, and regulated the long-distance transport and distribution of nitrate between roots and shoots in Arabidopsis. NRT1.5 and NRT1.8 homologous genes in B. rapa, B. oleracea, and B. napus were identified by bioinformatics with the basic sequences of AtNRT1.5 and AtNRT1.8 and analyzed in gene structures and proteins molecular ...