期刊论文

Cloning and characterization of phytochrome interacting factor 4 (BnaPIF4) gene from Brassica napus L

Guan, C.-Y.

中文

作物学报

0496-3490

2019

45

2

204-213

10.3724/SP.J.1006.2019.84085

本研究由国家重点基础研究发展计划(973计划)项目(2015CB150206)资助。 This study was supported by the National Basic Research Program (973 Program) (2015CB150206). * 通信作者(Corresponding author): 官春云, E-mail: guancy2011@aliyun.com 第一作者联系方式: E-mail: 812298771@qq.com Received(收稿日期): 2018-06-21; Accepted(接受日期): 2018-08-20; Published online(网络出版日期): 2018-09-25. URL: http://kns.cnki.net/kcms/detail/11.1809.S.20180920.1555.002.html

本校为第一机构

光敏色素互作因子4 (phytochrome interacting factor 4, PIF4)是光信号途径关键转录因子, PIF4与BZR互作介导光信号与油菜素内酯信号互作,参与植物光响应。本文在甘蓝型油菜湘油15号中克隆到2个PIF4基因,分别定位于A03号染色体和C03号染色体,命名为BnaPIF4_A03和BnaPIF4_C03,全长编码序列(coding sequence, CDS)、全长mRNA和全长基因分别为1242 bp和1245 bp、1701 bp和1731 bp、2527 bp和2665 bp,各自编码413和414个氨基酸。BnaPIF4_A03具有7个外显子和6个内含子, BnaPIF4_C03具有8个外显子和7个内含子,与测序品种中双11号相比, BnaPIF4_A03基因第1内含子存在单碱基插入突变,第4和第6内含子存在...

Phytochrome interacting factor 4 (PIF4) is a key transcription factor in light signaling pathway of plants, PIF4 interacts with Brassinazole-resistant (BZR) to mediate the interaction between light signal and brassinosteroid signal and participates in plant photoresponse. In this study, two novel PIF4 gene were isolated from Brassica napus L. cv. Xiangyou 15, they were identified on chromosomes A03 and C03 and encoding 413 and 414 amino acids, respectively, named as BnaPIF4_A03 and BnaPIF4_C03, their coding sequence (CDS), full-length mRNA and ...