期刊论文

Identification and function analysis of AVP1, VHA-a2, and VHA-a3 genes in Brassica napus L

Zhang, Z.-H.

中文

作物学报

0496-3490

2019

45

8

1146-1157

10.3724/SP.J.1006.2019.84124

本研究由国家重点研发计划项目(2018YFD0200901, 2018YFD0200906)和国家现代农业产业技术体系建设专项(CARS-13)资助。 This study was supported by the National Key R&D Program of China (2018YFD0200901, 2018YFD0200906) and the China Agriculture Research System (CARS-13). * 通信作者(Corresponding author): 张振华, E-mail: zhzh1468@163.com 第一作者联系方式: E-mail: yjy950606@163.com Received(收稿日期): 2018-09-20; Accepted(接受日期): 2019-04-15; Published online(网络出版日期): 2019-04-30. URL: http://kns.cnki.net/kcms/detail/11.1809.S.20190429.1518.002.html

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液泡是调控植物细胞分化、生长发育的重要部位, AVP1、VHA-a2和VHA-a3基因调控植物液泡内外离子平衡、离子运输以及能量供应。本研究利用功能已知的拟南芥AVP1、VHA-a2和VHA-a3基因为参考序列在甘蓝型油菜全基因组数据库、NCBI植物基因组注释数据库等鉴定并筛选出9个BnaAVP1、3个BnaVHA-a2和4个BnaVHA-a3,并分析基因拷贝数变异、分子特征、跨膜结构域、保守基序、染色体定位、系统进化树构建、蛋白二级结构及三维结构预测、高通量转录组测序等。发现甘蓝型油菜BnaAVP1和BnaVHA-a3的基因数量明显多于甘蓝和白菜;甘蓝型油菜AVP1、VHA-a2和VHA-a3蛋白属于由酸性氨基酸组成的稳定蛋白;系统进化选择能...

Vacuoles play an important role in regulating plant cell differentiation and growth. AVP1, VHA-a2, and VHA-a3 genes are important carrier elements for regulating the acid-base balance inside and outside the vacuole of plants and the energy provided by ion transport. In this study, nine BnaAVP1, three BnaVHA-a2, and four BnaVHA-a3 genes were identified in Brassica napus genome database and NCBI plant genome annotation database by using the known AVP1, VHA-a2, and VHA-a3 genes of Arabidopsis thaliana as reference sequences. Bioinformatics studies...