期刊论文

Mechanism of BnaBZR1 and BnaPIF4 regulating photosynthetic efficiency in oilseed rape(Brassica napus L.)under poor light

Guan, C.-Y.

中文

作物学报

0496-3490

2020

46

8

1146-1156

10.3724/SP.J.1006.2020.94198

本研究由国家重点基础研究发展计划(973计划)项目(2015CB150206)资助。 This study was supported by the National Basic Research Program of China (973 Program) (2015CB150206). * 通信作者(Corresponding author): 官春云, E-mail: guancy2011@aliyun.com 第一作者联系方式: E-mail: 812298771@qq.com Received (收稿日期): 2019-12-16; Accepted (接受日期): 2020-03-24; Published online (网络出版日期): 2020-04-03. URL: http://kns.cnki.net/kcms/detail/11.1809.S.20200403.0932.002.html

本校为其他机构

甘蓝型油菜品系XY881和XY883是湘油15辐照诱变后连续自交筛选的2个种子含油量、光合效率和弱光敏感性等有明显差别的子代品系。分别从XY881和XY883中克隆了芸薹素唑抗性因子1（brassinazole-resistant1,BnaBZR1）和光敏色素互作因子4 （phytochrome interacting factor 4, BnaPIF4）基因并进行了序列结构、表达和功能分析。结果表明, XY883的BnaBZR1和BnaPIF4基因存在结构变异,引起表达和调控模式的差异。XY883中BnaBZR1的启动子具有124 bp的富含A/T的插入序列,且XY883具有比XY881高的BnaBZR1表达,并且在弱光和2,4-表油菜素内酯（2,4-BL）诱导下具有较少的表达变化。XY883中BnaPIF4的5’-UTR区域...

Double-low seed rape B. napus L. varieties XY881 and XY883 screened from the same parent Xiangyou 15 have significant differences in seed oil content, photosynthetic efficiency, and poor light sensitivity. Brassinazole-resistant 1 (BnaBZR1) and phytochrome interacting factor 4 (BnaPIF4) genes were cloned from XY881 and XY883, respectively, and their sequence structure, gene expression and gene function were analyzed. There were structural mutations in the BnaBZR1 and BnaPIF4 of XY883, causing differences in gene expression and regulation. The p...